Yes...when they pick up different smells at the vets, they lose their recognizable smells and 'become a different kitty'. You can help alleviate the problem by taking a towel and rubbing it on all of the kitties that stayed home, then on the one that was spayed...and then again on the stay at home kitties. This will intermingle all their smells. You can also put a drop of vanilla extract on the back of all their necks. I'd do both...
Edit to add: It may take a day or two to calm down, even if you do what I recommended.
Welcome to the forum, we'd love to hear about your kitties and you in the Say Meow forum...pics would be great too!
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Holly, Misty & their friend, Jake, the dog.
Onyx, Callie May, Maggie & Kobi forever in my heart.
Yes, I know I need a new signature pic...someday...