i've seen laurief's recommendation many times to split it up into 250 mg 2X a day if you're giving that much, but that was after i'd been doing the whole 500mg at once for a long time already. so is it bad to do that? does she not absorb that much at one time or something? i did try splitting it up for a while, but then she stopped giving me nice poops daily. she skips days here and there. don't know if the lysine change had anything to do with it. i know it coulda been anything, of course.